Methods

For our research, we frequently employ the following techniques:

• in vitro cell culture experiments with lymphatic endothelial cells and leukocytes
  
• Fluorescence-Activated Cell Sorting for cell analysis and isolation (ETH Flow Cytometry Core Facility)
  
• in vivo experiments in mice (models of inflammation and of leukocyte trafficking)
  
• immunofluorescence / immunohistochemistry
  
• standard biochemical and molecular biology techniques, such as
  qRT-PCR, siRNA technology, gene overexpression, Western blot, ELISA
  
• transcriptomics analysis, in collaboration with the external page FGCZ
  
• confocal and multiphoton intravital microscopy, in collaboration with the ETH SCOPEM